Procedure :
Part I Prepared slides (whole mounts) Identify interphase and the stages of the mitosis on onion root tip Part II Make slide from squashed onion preparation Identify interphase and the stages of the mitosis on onion root tip
Part I. Examine the whole mount slide(s) of mitosis and find the different phases. You should do this first so that when you make your own preparation of the onion slide later you will know what to look for because you will have already seen the different phases. Part II. In order to make your own preparation of mitosis in the onion root tip, you will learn a technique of staining cells undergoing different phases of mitosis. It will be up to you to find all the different stages. Follow the instructions on the handout. Part III. One of the best ways to learn mitosis is to simulate each of the different stages with a model. In this case the model will be pipe cleaners representing homologous chromosomes and plastic rings will be the centromere that binds homologous chromosomes together. Perform the simulation with two different homologous chromosomes and go through interphase, prophase, metaphase, anaphase and telophase with the pipe cleaners and beads.
Materials :
- Whole Mount
- Onion Slide
- Onion Root Tip
- Pipe Cleaners Plastic Rings
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